Description And Function Of Mutagenesis - 1345 Words

Site-directed mutagenesis is a method used to construct amino acid changes in a protein in order to test the function of a specific amino acid. It is the way to study protein structure and function change the amino acid sequence of a protein by altering the DNA sequence of cloned gene4. A particular amino acid is very important in catalytic activity, ligand binding, protein folding or other function. Amino acid residue significance is tested by making conservative substitutions or by changing the amino acid to either alanine or glycine. Site-directed mutagenesis is also used to construct compensatory mutations, which are used to show the importance of specific interactions by making changes in both interacting partners. Each change individual destroy the interaction and combination of changes restores the interaction. These type of experiments are typically between two protein or between protein and nucleic acid. Used site-directed mutagenesis to investigate the interaction between h Pol k and DNA1. DNA polymerase kappa is a Y-family polymerase that use to bypass the damage DNA as translesion synthesis polymerase. In prostate cancer has reported that it has higher frequency of mutation in DNA polymerase kappa. 28% of patients have somatic mutations in POLK gene of prostate tumor and dominated by C- to –T transition2. Approximately 30,000 DNA lesions occur per cell per day in mammalian cells. This damage to DNA can lead to cell death as well as disease such as cancer.Show MoreRelatedTopology of the Membrane-Bound 1069 Words   |  4 Pagescomplement protein C9 probed by glycosylation mapping, anti-peptide antibody binding, and disulfide modification Và ©ronique Rossia,b, Yunxia Wanga,1, Alfred F. Essera,âˆâ€" Introduction and background: Although the importance of human complement C9 in MAC1 function remains unchallenged, the exact mechanism by which C9 induces internalization of the MAC1 complex into pathogenic membranes is unclear. Since the early 1980s, multiple functional properties of C9 have been defined encouraging the creation of severalRead MoreThe Merkel Cell ( Mcc )1459 Words   |  6 PagesBackground: The Merkel Cell was identified in 1875 by Friedrich Sigmund Merkel, who was the president of the University of Rostock, as well as a professor and physician. Sigmund provided the first full description of Merkel cells and revealed that the merkel cell was a component of the touch receptor. (Halata et al., 2003) MCC was first reported in 1972 by Cyril Toker as â€Å"trabecular carcinoma of the skin† that was mainly found in elderly patients. (Journalof Skin Cancer. 2013). Soon after, They wereRead MoreThree Important Findings From This Study1414 Words   |  6 PagesAdenosine-to-inosine RNA editing Promotes cancer progression and therapeutic resistance by contributing to LSC self-renewal Activated by JAK2 signaling E. LIN28b/Let-7 axis The expression of let-7 family is required for developmental timing and tumour suppressor function, but must be suppressed for the self-renewal of stem cells (3). Biogenesis of let-7 is regulated by LIN28b (3). Findings in the article suggest that JAK2 signaling â€Å"increases LIN28B pluripotency gene expression and inhibit the expression of let-7Read More P-type ATPases Essay2118 Words   |  9 Pagesis the transport of sodium and potassium ions across the cell membrane. It is this Na+/K+ ATPase that J.C Skou discovered, and worked on for most of his academic career (Skou 1997). The fundamental basis of the P-type ATPases ability to function is its capacity to form 2 conformational states, E1 and E2. Both of these states are ion-binding, one allowing intramembrane ion binding, and the other with an extramembrane ion binding site. The Na+/K+ ATPase is an anti-porter, transporting Na+Read MoreSmall Molecule Of Voltage Gated Sodium Channels4776 Words   |  20 Pagescomplex nanomachines and the details of their workings have only recently started to become clear. Here we review  ¬Ã‚ ¬Ã¢â‚¬â€œ with emphasis on the computational studies – some of the major milestones in the long-standing search of a quantitative microscopic description of the molecular mechanism and modulation of voltage-gated sodium channels. Physiological Role of Voltage Gated Sodium Channels (VGSCs) To respond to changes in the external environment, cells propagate electrical signals generated by transient